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. 2016 Mar 19;5(6):379–391. doi: 10.1016/j.molmet.2016.03.003

Figure 3.

Figure 3

PI3K pathway is required for LPS induced hypophagia. Top panel (A) showing representative photomicrographs of pAKT T308 expression (green) in the hypothalamic arcuate (ARC) nucleus 2 h after saline or LPS (100 μg/kg, ip) injection, in LepR reporter mice (red). 3V = third ventricle. Scale bar: 50 μm. Inset images show examples of LepR neurons co-expressing or not pAKT, 40× magnificent. Graphs showing 2, 14 and 24 h food intake (B) and 24 h changes in body weight (C) in wild type mice treated with icv injection of vehicle (2% DMSO in saline, 3 μl) or PI3K inhibitor LY294002 (1 μg/mouse, 3 μl), followed by saline or LPS (100 μg/kg, ip) injection. Two-way ANOVA followed by Tukey's post hoc test was performed. Data are expressed as mean ± SEM. (n = 8). *p < 0.05: Vehicle + Saline vs Vehicle + LPS; a p < 0.05: Vehicle + LPS vs LY294002 + LPS.