Figure 4.

Validation of deletion of PI3K p110α catalytic subunit in LepR cells. The percentage of relative mRNA expression in punches from the hypothalamic arcuate nucleus (ARC) of intact p110α ^loxP controls in comparison with LepR^Δp110α mice (A) (n = 6–7/group). The number of ARC pAKT positive cells in p110α^loxP and LepR^Δp110α mice 2 h after saline or LPS (100 μg/kg, ip) treatment (B) (n = 5/group). Representative photomicrographs of pAKT T308 expression (green) in the ARC of p110α^loxP and LepR^Δp110α mice 2 h after treatment (C). 3V = third ventricle. Scale bar: 50 μm 2-tailed Student's t test (A) and Two-way ANOVA followed by Tukey's post hoc test (B) were performed. Data are expressed as mean ± SEM. *p < 0.05: p110α ^loxPvs LepR^Δp110α and p110α ^loxP Saline vs p110α ^loxP LPS.