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. 2016 Mar 19;5(6):379–391. doi: 10.1016/j.molmet.2016.03.003

Figure 7.

Figure 7

Validation of deletion of PI3K p110α and p110β catalytic subunits in LepR cells and LPS-induced protein phosphorylation in the mediobasal hypothalamus. A: Percentage of relative mRNA expression in punches from the hypothalamic arcuate nucleus (ARC) of intact p110α + β loxP and LepRΔp110α+β mice. Percentage of pAKT (B), pFoxO1 (C) and pSTAT3 (D) expression in the mediobasal hypothalamus, 2 h after saline or LPS (100 μg/kg, ip) injection (n = 5 - 6/group). 2-tailed Student's t test (A) and Two-way ANOVA followed by Tukey's post hoc test (B–D) were performed. Data are expressed as mean ± SEM. (n = 8/group). *p < 0.05: p110α + βloxPvs LepRΔp110α+β and p110α + βloxP Saline vs p110α + βloxP LPS; a p < 0.05: p110α + βloxP LPS vs LepRΔp110α+β LPS; #p < 0.05: LepRΔp110α+β Saline vs LepRΔp110α+β LPS.