Figure 4.

Adoptive transfer of immune cells protects against HFD-induced insulin resistance and dysglycemia. (A–G) 3 × 10⁷ splenocytes from CD45.1 mice injected with PBS (■; naive splenocytes) or with the ileum contents from mice fed HFD (▲; treated splenocytes) were transferred into CD45.2 naïve recipients fed HFD, as summarized in (A). (B,E) Intraperitoneal GTTs were performed after one month (B) and two months (E) of HFD. Glucose tolerance indices were calculated (C,F). (D,G) Fasting plasma insulin was quantified (μg/ml). Data are means ± SEM; n = 8 mice per group. Data with similar superscript letters (a,b) indicate data non-statistically different between each other at a probability threshold p > 0.05. (H,I) Rag1-deficient mice (Rag1 ko) were injected s.c. with 200 μl of PBS (●) or with the ileum contents from mice fed the HFD (HFD treated; ■). Thirty-five days later, the mice were fed HFD. After one month of HFD, an intraperitoneal GTT (H) was performed and the glucose tolerance index (I) was calculated. Data are means ± SEM. n = 5 mice/group. Data with similar superscript letters (a,b) indicate data non-statistically different between each other at a probability threshold p > 0.05. The experiment was performed twice.