Fig. 5.

Relative expression of pCP13 genes in the virR and revR mutants compared to the wild type. a The expression levels of all plasmid-encoded genes in the virR (blue) and revR (red) mutants. Note that no FDR and fold-change cutoff criteria were applied to the data represented here. b Real-time PCR was performed to validate differentially expressed pCP13 plasmid genes in the virR and revR mutants. Expression levels were relative to the expression of the housekeeping gene, rpoA, and were the average of at least three independent biological replicates (n ≥ 3, mean ± SEM). The asterisk (*) denotes a statistically significant difference (p ≤ 0.05) as calculated with the student’s unpaired t-test