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Plasmids used to construct the overexpressed DXP pathway in E. coli Bl21 (DE3)
| Gene(s) | Vector | Multiple cloning site | Restriction sites |
|---|---|---|---|
| dxr | pCola-Duet-1 | I | NcoI, EcoRI |
| dxs | pCola-Duet-1 | II | NdeI, XhoI |
| ispD/ispF operon | pCDF-Duet-1 | I | NcoI, EcoRI |
| idi | pCDF-Duet-1 | II | NdeI, XhoI |
