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Plasmids used to compare the activity of CotB3 and CotB4 hydroxylases with different redox system variants
| P450 proteins | Redox system | Mutations |
|---|---|---|
| CotB3 | AfR/Afx | – |
| CotB3 | PdR/Pdx | – |
| CotB3 | PdR/Pdx | D38E |
| CotB3 | PdR/Pdx | W106E |
| CotB3 | PdR/Pdx | D38E, W106E |
| CotB4 | AfR/Afx | – |
| CotB4 | PdR/Pdx | – |
| CotB4 | PdR/Pdx | D38E |
| CotB4 | PdR/Pdx | W106E |
| CotB4 | PdR/Pdx | D38E, W106E |
