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. 2016 Mar 30;6:23671. doi: 10.1038/srep23671

Figure 3. The ROCK inhibitor Y-27632 produced complex impedance profiles, but showed a clear barrier-protecting effect in the MP assay.

Figure 3

HMECs were treated with the ROCK-inhibitor Y-27632 (10 μM) for 30 min and subsequently with TRAP (50 μM). Representative experiments show time-dependent alterations of CI (A) or impedance values (B), both normalized to untreated cells (control). The bar diagrams show the statistical analysis of all experiments for CI (A) or impedance changes (B) at time points indicated by black arrows (1: TRAP addition; 2: maximum effect of TRAP; 3: after 3.5 h) in the corresponding experiments. (C) Macromolecular permeability of FITC-dextran (40 kDa) across an HMEC cell layer was measured with a Transwell® two-compartment system. Samples were taken from the lower compartment at indicated time points. The results are depicted as normalized FITC-dextran concentrations or as absolute permeability (reference time point was 60 min in each case). All experiments were performed independently at least three times. Data are expressed as mean ± SEM. *P ≤ 0.05 (one-way ANOVA followed by Newman-Keuls post-hoc test).