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Transient production of FVIIIΔB and FVIIIΔB-F309S in HEK 293 cells. The factor FVIII production was measured by the enzyme-linked immunosorbent assay (ELISA) technique. aStatistically significant differences between groups using Student's unpaired t-test (FVIIIΔB: p-value = 0.0042; FVIIIΔB-F309S: p-value = 0.0009).
