Fig. 3.

Suramin pretreatment reduces inflammation in the kidney after cisplatin treatment. C57BL/6J mice were pretreated with suramin (10 mg/kg) or an equal volume of vehicle via tail vein injection 72 h before cisplatin administration (20 mg/kg ip). Mice were euthanized 72 h after cisplatin administration, and the relative expression of TNF-α (A), IL-6 (B), chemokine (C-X-C) ligand (CXCL)1 (C), monocyte chemotactic protein (MCP)-1 (D), and IL-1β (E) was measured via qRT-PCR. V indicates vehicle control, S10 indicates suramin iv pretreatment at 10 mg/kg, C indicates cisplatin ip at 20 mg/kg, and CS10 indicates suramin iv pretreatment at 10 mg/kg followed by cisplatin ip at 20 mg/kg 72 h later. Data are means ± SE. *P ≤ 0.05 compared with the cisplatin-treated group as determined by two-way ANOVA.