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. 2016 May 23;16:92. doi: 10.1186/s12866-016-0703-x

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© Watcharakul et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Assays for oxidative polyisoprene cleavage. (a) Oxygen consumption assay: 4 μg of the indicated enzyme (control without enzyme) was added after 3.75 min (arrow) and oxygen saturation was recorded. Activities were calculated from the slopes of the graphs during the first minutes. The mixtures were extracted with ethylacetate after 90 min of incubation at 23 °C and the formed cleavage products were analysed by HPLC (b). ODTD was the main cleavage product of RoxA_Xsp but was present only in trace amounts in Lcp_Rr and Lcp_K30 preparations. The graph for Lcp_Rr is given in its inverse orientation. Assays with each enzyme were performed at least three times; one typical dataset is shown