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. 2016 May 23;16:92. doi: 10.1186/s12866-016-0703-x

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© Watcharakul et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — pH optimum of Lcp_Rr. The pH optimum was determined using the HPLC-based product assay in a pH range of 5 to 11 using acetate buffer (pH 5 - pH 6, diamonds), phosphate buffer (pH 6 - pH 9, open circles), carbonate buffer (pH 9 – pH 11, squares), or HEPES (pH 7, closed circle). Assays were performed with two biological and two technical replicates. Error bars indicate standard deviation