Table 1.
Properties of biochemically characterized rubber oxygenases
| LcpRr | LcpK30 | LcpVH2 a | RoxAXsp | |
|---|---|---|---|---|
| Gene length [bp] | 1227 | 1224 | 1224 | 2037 |
| Residues (pre-/mature enzyme) | 408/378 | 407/377 | 407/371a | 678/648 |
| Molecular mass (apo-/mature protein) [kDa] | 45.2/42.2 | 44.0/41.0 | 45.5/41.7a | 74.7/71.5 |
| strep tagged [kDa] | 44.5 | 43.3 | ||
| pH optimum | 8 | 7 | 7 | 7 |
| Molar extinction coefficient [104 M−1 cm−1] | 9.5 (407 nm) | 8.0 (412 nm) | n.d. | 20.6 (406 nm) |
| Metal atoms per protein molecule | ||||
| Fe | 0.98 | 1.16/1.05 | n.d. | 2.3 |
| Cu | 0.36 | 0.056/- | sub-stoichiometric | - |
| Mn | - | - | n.d. | - |
| Ni | - | 0.12/0.05 | n.d. | - |
| Zn | - | - | n.d. | - |
| Mg, Ca | - | n.d. | n.d. | n.d. |
| Haeme type | b-type | b-type | not known/no haeme | c-type |
| Oxidation state of haeme iron | Fe3+ | Fe3+ | Fe3+---O2- | |
| UVvis effect upon addition of CO | no | no | yes | |
| Specific activity [U/mg] (23/30/37 °C) | 0.9/3.1/b | 1.5/n.d./4.6 | 1.3/n.d./n.d. | n.d./0.48/n.d. |
| Melting point | n.d. | 61.5 °C | n.d. | 54.3 °C |
| Conserved DUF2236 residuesc | ||||
| R | 163 | 164 | 161 | |
| T | 167 | 168 | 165 | |
| H | 197 | 198 | 195 |