Fig. 4.

Exogenous H₂S attenuated autophagy by inhibiting activation of AMPK signaling pathway. a The expression of p-AMPK and AMPK was detected by Western blotting. The OD value of P-AMPK was standardized to that of AMPK. There data were summarized from at least three different experiments. (**p < 0.01 vs control group. ^##p < 0.01 vs HG + Pal group). b Compound C inhibited autophagy induced by HG + Pal. The expression of LC3II/I, Atg7, P62 and lamp2 was examined by Western blotting. Each OD value was standardized to that of β-actin. There data were summarized from at least three different experiments (*p < 0.05 vs control group. **p < 0.01 vs control group. ^#p < 0.05 vs HG + Pal group. ^##p < 0.01 vs HG + Pal group)