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. Author manuscript; available in PMC: 2016 May 24.
Published in final edited form as: Chem Biol. 2013 Jul 25;20(7):912–921. doi: 10.1016/j.chembiol.2013.05.010

Figure 4. PIKfyve modulates TLR-induced IL12p40 expression.

Figure 4

(A) THP-1 cells expressing indicated shRNAs were lysed and blotted with indicated antibodies. (B) THP-1 cells expressing indicated shRNAs were stimulated with IFNγ (50 ng/ml)/LPS (1 µg/ml) overnight. The cytokine production was measured by ELISA. **, P<0.01 using Student’s t-Test, indicating a significant difference on IL12p40 production between cells expressing control (NT) and PIKfyve shRNA. (C) Images of BMDCs from wild-type (WT) and ingls mice and those from WT BMDCs treated with DMSO or apilimod (1 µM). (D) BMDCs from WT or ingls mice were challenged with IFNγ (50 ng/ml)/LPS (1 µg/ml) or R848 (0.1 µM). The cytokine production was measured by ELISA following overnight stimulation. Representative results from three independent experiments. **, P<0.01 using Student’s t-Test, indicating a significant difference between the samples from WT and ingls mice with the same treatment.