Figure 1. Haploinsufficiency of Meox2 expression causes reduction in microvascular density.
(A) Western blot analysis showing a significant reduction (48%) of MEOX2 protein in B6.Mx−/+ mice. (B) Representative images and quantitative analysis of CD31 immunostaining in the cortex of B6 and B6.Mx−/+mice at 4 months of age, demonstrating a significant reduction in microvascular density. (C) Representative images and quantitative analysis of NEUN immunostaining in the cortex of B6 and B6.Mx−/+ mice at 10 months of age. No changes in the number of neurons were found between groups. In (A-C) values are relative mean ± SEM to the B6 values, n= 4 mice per group, *P= 0.0406, and ***P= 0.0008 by unpaired t-test. Scale bars: 50μm.