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. 2016 May 24;8:20. doi: 10.1186/s13195-016-0186-x

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© Delay et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — In silico approach for assessing score thresholds for identification of microRNA (miRNA) target sites and polymorphisms in a microRNA target site (PolymiRTSs). a A graphical representation of the “basic score filtering” in TargetScan. The “context +” scores for each miRNA binding site are plotted on the x-axis, and the number of counts with which each score was found is plotted on the y-axis. The top 5 % context + scores had a value below −0.318. b A graphical representation of the “score difference filter” in TargetScan. The percentage change in the context + scores for each miRNA binding site (due to the presence of a PolymiRTS) is plotted on the x-axis, and the number of counts with which each change in score was found is plotted on the y-axis. The top 5 % context + score changes had a value below −8.25 % or above 8.25