Fig. 7.

Quantitative analysis of IgG clearance. Signals from the blots (HC and LC) in Fig. 6a and c were quantified and normalized with their corresponding tubulin signals and used to plot their clearance curves in (a) and (b), respectively. The data represents the average of two or three blots normalized for loading differences using an anti-tubulin blot. There is a clear variation between HC and LC clearance for all strains and also between the strains in both of the plots. The data gives a general indication that a constant pool of available LC might be beneficial for efficient IgG folding and secretion