Table 1.
Strain number | Added elements | Strain | Specific product yield (µg ml−1OD600−1) | Relative frequencya | P valueb | Fold-change relative to wild-typec |
---|---|---|---|---|---|---|
154 | CPR5-P GPD | Δopi1 | 0.2027 | 0.2 | 0.000002 | 10.22 |
157 | CPR5-P KAR2 | Δopi1 | 0.1992 | 0.15 | 0.000159 | 10.15 |
155 | CPR5-P GAL1 | Δopi1 | 0.1579 | 0.1 | 0.000560 | 7.99 |
153 | CPR5-P TEF | Δopi1 | 0.1525 | 0.075 | 0.000036 | 7.66 |
156 | CPR5-P PDI1 | Δopi1 | 0.1424 | 0.05 | 0.007624 | 7.15 |
170 | ERO1-P GAL1 | Δopi1 | 0.1226 | 0.05 | 0.020502 | 6.13 |
212 | SIL1-P GAL1 | Δopi1 | 0.1223 | 0.05 | 0.021128 | 5.53 |
162 | PDI1-P TEF | Δopi1 | 0.1208 | 0.075 | 0.279237 | 6.09 |
206 | LHS1-P GPD | Δopi1 | 0.1163 | 0.05 | 0.299870 | 5.88 |
402 | LHS1-P KAR2 + SIL1-P KAR2 | Δopi1 | 0.1139 | 0 | 0.011316 | 5.73 |
83 | None | Δopi1 | 0.0886 | 0.075 | 4.47 | |
73 | None | wt | 0.0199 | 0 | 1 |
The data represent the mean value of the experiments conducted with 0.5 and 2 % galactose induction at 30 °C
aRelative frequency in the 95th percentile of Δopi1-strains
bCalculated with the nonparametric equivalent of t test, Wilcoxon signed rank test using the Δopi1-strain without added elements as the reference
cAverages of the fold-changes relative to wild-type