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. 2016 May 23;17:390. doi: 10.1186/s12864-016-2739-6

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Shows a significantly high linear correlation (r² = 0.8832, P < 0.0001) between RNA-seq and quantitative real time PCR (qRT-PCR) data for the TO-VS-IFN/SAV3 and TO-VS-SAV3. Dotted lines show the 95 % confidence limits for the correlation coefficient (r²) slope (95 % CI 0.8478 - 0.9771). qPCR data was generated as the mean of the fold increase in gene expression relative to the β-actin control group from three independent replicates (N = 3) of TO-cells simultaneously treated with type I IFN and infected with SAV3 (TO-VS-IFN/SAV3) together with none-IFN treated cells infected with SAV3 (TO-VS-SAV3). The cells used in this study were independent of cells used for RNA-seq data analysis