Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 23;213(4):435–450. doi: 10.1083/jcb.201501120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 3. — Asl regulates the position of centriole proteins. (A–E) Centrioles from interphase wt (asl^mecD/TM6B) and asl mGSCs or wt mature SCs. (A) GFP::Sas-6 (green) and Ana1::tdTomato (red). Sas-6 extends the length of the centriole in asl mGSCs but is only proximal in wt SC centrioles (brackets). (A′) Length of Sas-6 signal in wt SCs (1) and asl mGSCs (2). (B) Sas-4::GFP (green) and Ana1::tdTomato (red). In SCs, Sas-4::GFP localizes proximally (bracket), where endogenous protein localizes (Gopalakrishnan et al., 2011; Fu and Glover, 2012). Additional localization beyond Ana1 (asterisk) is a consequence of overexpression. In asl mGSCs, Sas-4 is uniformly along the entire length of Ana1. (B′) Lengths of the Sas-4 signal in wt SCs (1, only the proximal signal) and asl mGSCs (2). (C) The radial distribution (diameter) of centriole proteins is unaltered in asl mGSCs. (D) GFP::Cep97 (green) and Ana1::tdTomato (red). (D′) Intensity of GFP::Cep97, normalized to the mean intensity of Cep97, at centrioles in wt (1) and asl (2) mGSCs. Cep97 levels are unaffected in asl. (E) CP110::GFP (green) and Ana1::tdTomato (red). (E′) Intensity of CP110::GFP, normalized as in D, in wt (1) and asl (2) mGSCs. CP110 levels are unaffected in asl. Bars, 1 µm. Mean ± SD, red. Comparison by unpaired t tests, with Welch’s corrections when appropriate. ****, P < 0.0001; n.s., not significant.