Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 May 23;213(4):435–450. doi: 10.1083/jcb.201501120

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 5. — Asl is not required for PCM recruitment during mitosis. (A–E) Centrioles (Ana1::tdTomato, red) from mitotic wt (asl^mecD/TM6B) and asl mGSCs or wt mature SCs. Asterisk represents centrioles in adjacent hub cells. (A) PLP (green) localizes to centrioles in wt mGSCs, wt SCs, and asl mGSCs (blue box, n = 7/7). mGSC centriole, arrowhead. DNA, d (phosphohistone, not depicted). (B) Cnn (green) localizes to centrioles in wt mGSCs, wt SCs, and asl mGSCs (blue box, n = 3/3). (C) Spd2 (green) localizes to centrioles in wt mGSCs, wt SCs, and asl mGSCs (blue box, n = 4/4). (D) γ-Tubulin (green) localizes to centrioles in wt mGSCs, wt SCs, and asl mGSCs (blue box, n = 10/10). (E) MTs (green) organized around centrioles in wt mGSCs, wt SCs, and asl mGSCs (blue box, n = 8/8). DNA, d (phosphohistone); actin, a (phalloidin). (F) Frames from Videos 1 and 2 of mGSCs in wt (top row) and asl (bottom row) expressing GFP::α-tubulin (MTs, green) and Ana1::tdTomato (red). Time (min) relative to nuclear envelope breakdown (NEB; red box). Anaphase onset (AO; purple box). (G) γ-Tubulin (green) localizes to centrioles in meiotic asl SCs (blue box, n = 15/15). Asl is absent from centrioles in asl (bottom right). Bars: (A–E and G) 1 µm; (F) 5 µm.