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. 2016 May 23;213(4):435–450. doi: 10.1083/jcb.201501120

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Figure 6. — Cep97 controls mGSC centriole length. (A–D) mGSCs in adult testes of indicated genotypes. Ana1::tdTomato, red (in A); PLP, red (in B–D); phalloidin, green; DAPI, blue. Arrowheads represent centrioles in mGSCs. Asterisks represent centrioles from the hub. (A) An mGSC from a cp110Δ testis with a long centriole. The other centriole is not visible in this projection. (B) cep97^LL/Df(2L)A1 (LL/Df) mGSC with a long apical centriole. (C) LL/Df mGSC with a long nonapical centriole. (D) An LL/Df mGSC expressing GFP::Cep97 has normal-length centrioles. (E) Percentage of mGSCs with long centrioles per testis ± SEM for indicated genotypes. Testes counted include cp110Δ, 36; LL/CyO, 17; LL/LL, 34; LL/CyO; GFP::Cep97, 23; LL/Df, 32; and LL/Df; GFP::Cep97, 27. Comparison: unpaired t tests, with Welch’s correction. ****, P < 0.0001; ***, P < 0.001; *, P ≤ 0.05; n.s., not significant. (F) GFP::Sas-6 (green) and PLP (red) extend along the length of cep97 centrioles. Procentriole can position at one end (middle row) or the middle (bottom row) of the elongated centriole. (G) Remnant centrioles (Asl, green; PLP, red) in cep97;plk4 double-mutant adult mGSCs are longer than in wt (yw) or plk4. mGSCs, orange line; DAPI, blue; arrowheads, centrioles in mGSCs. Boxed region enlarged (bottom). (H) Centriole (Ana1::tdtomato) length (red line, mean ± SD) in mGSCs in adults of the indicated genotypes. Dashed line is the mean of wt plus two SDs. Almost all centrioles in mGSCs are long in cep97;plk4. Comparison: ordinary one-way analysis of variance followed by Tukey’s multiple comparisons test. ****, P < 0.0001; n.s., not significant. Bars: (A–D) 2 µm; (F and G, bottom row) 1 µm; (G) 5 µm.