Table 1. Plus end dynamic instability parameters for full-length wild-type tubulins and chimeric tubulins.
| Total tubulin concentration and recombinant protein | Polymerization rate (subunit · s−1) | Catastrophe frequency (min−1) | ||
|---|---|---|---|---|
| Mean ± SD | n | Mean ± SD | n | |
| 6 μM | ||||
| α/βIIB | 9 ± 2 | 70 | 0.06 ± 0.008 | 60 |
| α/βIIB-tail-III | 6 ± 1 | 87 | 0.08 ± 0.008 | 92 |
| α/βIII-tail-IIB | 8 ± 2 | 37 | 0.12 ± 0.02 | 58 |
| α/βIII* | 8 ± 3 | 77 | 0.10 ± 0.01 | 105 |
| 10.5 μM | ||||
| α/βIIB | 15 ± 3 | 70 | 0.03 ± 0.006 | 33 |
| α/βIIB-tail-III | 14 ± 3 | 81 | 0.03 ± 0.005 | 37 |
| α/βIII-tail-IIB | 17 ± 2 | 88 | 0.10 ± 0.01 | 115 |
| α/βIII* | 16 ± 6 | 75 | 0.10 ± 0.01 | 104 |
| Mixed (3:1) (α/βIIB:α/βIII) | ND | 0.05 ± 0.008 | 41 | |
| Mixed (1:1) (α/βIIB:α/βIII) | 14 ± 2 | 79 | 0.06 ± 0.007 | 65 |
| Mixed (1:3) (α/βIIB:α/βIII) | ND | 0.12 ± 0.009 | 153 | |
| 13 μM | ||||
| α/βIIB | 25 ± 4 | 52 | 0.03 ± 0.006 | 20 |
| α/βIIB-tail-III | 21 ± 3 | 49 | 0.008 ± 0.003 | 9 |
| α/βIII-tail-IIB | 19 ± 3 | 53 | 0.08 ± 0.01 | 62 |
| α/βIII* | 19 ± 4 | 57 | 0.09 ± 0.01 | 68 |
Measurements are from data presented in Fig. 2 (F, G, N, and O) and Fig. 4 (I and J). Mean ± SD are shown. n represents the number of filaments analyzed (for polymerization rate) or catastrophe events (for catastrophe frequency). *, data from Ti et al. (2016); ND, not determined.