Table 2.

Cellular Effects of Peptidoglycan Derivatives on Nod2 Signaling and Stability^a

Compound	NF-κB Fold Activation ± SD	Half-life (h)± SD
Control	No activation	7.1 ±1.4
1	1.8 ±0.3	15.9 ±4.1*
2	3.1 ±0.3	23.1 ±2.2*
3	No activation	7.7 ±0.7
8	2.2 ±0.3	Not tested
9	2.6 ±0.4	10.5 ±2.6*
10	1.9 ±0.3	15.0 ±1.8*
11	2.3 ±0.3	Not tested
12	2.4 ±0.3	11.7 ±2.7*
13	1.8 ±0.3	8.9 ±0.9*
15	2.5 ±0.3	Not tested
16	No activation	8.0 ±0.7
18	2.8±0.119	19.0 ±1.8*

^a(+) Nod2 plasmid NF-κB activation normalized to the (−) Nod2 plasmid. Nod2 band intensities were plotted against time assuming first-order decay (ln(I_r) vs time). The rate constant was calculated using the negative slope of the line (k = −slope), and the corresponding half-life was calculated (T_1/2 = ln(2)/k).²¹ SD = standard deviation.

^*= P < 0.05, stabilizes compared to the untreated control. The control, 3, and 16 did not significantly activate.