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Validation of the shortening of survivin 3'-UTR by 3' RACE. Lane 1: 2-log DNA ladder. Lane 2: 3' RACE nested PCR product of NFT specimen. Lanes 3–6: 3' RACE nested PCR product of SOC samples with varying coding region expression levels and proximal/distal ratios; 3'-UTR: 3'-untranslated region; 3' RACE: Rapid amplification of cDNA 3' ends. SOC: Serous ovarian cancer; PCR: Polymerase chain reaction; NFT: Normal fallopian tube.
