Fig. 1.
Structural analysis of products formed by the oxygenation of 2-AG ether by huPGHS-2. A: RP-HPLC separation and electrospray (positive ions) MS of reaction products of 2-AG with huPGHS-2. Peaks labeled A (blue), B (red), and C (green) were found to have molecular ions [M+NH4]+ at m/z 642, 524, and 466, respectively. B: Collisional activation of m/z 642 (peak A). Structure inset with origin of major product ion indicated as the acetate derivative. C: Collisional activation of m/z 524 (peak B). Structure inset of proposed metabolite indicated as the acetate derivative. D: Collisional activation of m/z 466 (starting material, peak C). Structure inset of proposed monoacetate adduct indicated.