Fig. 4.

Comparison of the effects of nsFAs on the oxygenation of 2-AG and 2-AG ether by huPGHS-2. Measurements of COX activity were performed in a standard O₂ electrode assay with 5 μM 2-AG (A) or 5 μM 2-AG ether (B) as the substrate in the presence or absence of 25 μM PA (16:0), stearic acid (18:0), oleic acid (18:1ω9), or eicosaenoic acid (20:1ω9), as described in the Experimental Procedures. The same amounts of enzyme protein were used in all assays, and the value without a nsFA was normalized to 100%. Results are shown for a single experiment involving triplicate determinations. The error bars indicate the average ± SD. Asterisks denote significant differences from the value without a nsFA, as determined by the Student’s t-test (P < 0.03).