Fig.4.

Tangles of dystrophic myelinated axons in cortex after LPS/IS/H. Myelin basic protein (MBP) immunostaining of cortex ipsilateral to the focal ischemia at 2 weeks (B), 4 weeks (A), 8 weeks (C), and 12 weeks (D) following LPS/IS/H. Note a tangle of enlarged MBP immunostained fibers that are focal and disorganized (A-D, white arrow). MBP staining of axons perpendicular and parallel to the cortical surface is observed which appears to be normal (A, figure from ipsilateral hemisphere but similar findings bilaterally). MBP immunostaining of the fibers in the tangle (E) co-localizes with axonal neurofilament light chain protein (NF-L) (F) as shown by the orange fibers in the merged image (G). These data suggest the tangles are composed of enlarged fibers that appear to be enlarged myelinated axons that are focal and disorganized. Bar = 50 μm.