Fig.5.

Localization of N-SMase2 in cortex of control rat and in rats 4 weeks and 12 weeks following LPS/IS/H. In control, N-SMase2 was localized to occasional cell bodies and to occasional axons, with N-SMase2 and neurofilament light chain protein (NF-L) co-localizing in these axons (N-SMase2/NF-L). At 4 weeks post LPS/IS/H, N-SMase2 was localized to large numbers of abnormal linear structures and some of these structures co-localized with NF-L (N-SMase2/NF-L). By 12 weeks post LPS/IS/H, N-SMase2 was again observed in a few scattered axons in cortex that co-localized with NF-L and appeared similar to controls. Bars = 50 μm.