Fig.9.
Co-localization of myelin aggregates with rodent Aβ and Aβ1 - 42 expression in the hemisphere ipsilateral to ischemia at 12 weeks following LPS/IS/H. A) Myelin aggregates stained with myelin basic protein (MBP) co-localized with rodent Aβ (R Aβ) deposits. Most of the MBP stained foci co-localized with rodent Aβ (white arrows, Merge) whereas some did not (yellow arrows). R Aβ likely represents AβPP. B) Myelin associated glycoprotein (MAG) co-localized with Aβ1 - 42 deposits. MAG immunostained foci in cortex co-localized with Aβ1 - 42 (B, Merge). Bars = 50 μm. C) Western blot analysis for Aβ1 - 42 at 12 weeks following saline/IS/H and LPS/IS/H. Two bands of ∼150 kDa and ∼140 kDa were detected on Western blots using the Aβ1 - 42 antibody which showed marked induction of both bands following LPS/IS/H compared to saline/IS/H. D) Quantification of the expression of Aβ1 - 42 showed that it markedly increased following LPS/IS/H compared to that following saline/IS/H. **p < 0.01.