Figure 5.

IKKβ protects adipocytes from HFD-induced cell death. A: Representative TUNEL staining of epiWAT sections from ND- or HFD-fed IKKβ^F/F and IKKβ^ΔAd mice. The nuclei were stained with DAPI (blue), and the TUNEL-positive cells were indicated by arrows. Scale bar, 50 μm. B: Representative immunohistochemistry for perilipin of epiWAT sections from ND- or HFD-fed IKKβ^F/F and IKKβ^ΔAd mice. The nuclei were stained with hematoxylin (blue), and the perilipin-negative adipocytes were indicated by arrows. Scale bar, 100 μm. C: Western blot analysis with the indicated total and phosphorylated (p) antibodies in epiWAT from ND or HFD-fed IKKβ^F/F and IKKβ^ΔAd mice.