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. 2016 Mar 18;65(6):1521–1533. doi: 10.2337/db15-0580

Figure 3.

Figure 3

Hypoxia with iron deficiency impairs hepatic glucose production despite no change in the expression of gluconeogenic genes in mouse liver. For pyruvate (A) or alanine (C) tolerance testing, mice were fasted for 16 h, followed by an intraperitoneal injection of sodium pyruvate or L-alanine (2 g/kg body weight). B and D: The area under the glucose curve was calculated for the studies in A and C. Relative mRNA abundances of PEPCK (E) and G6Pase (F), normalized to levels of RPL13a, were determined by quantitative RT-PCR in mouse liver. Values are expressed as mean ± SE (n = 4–5 per group). ALT, alanine tolerance test; PTT, pyruvate tolerance test. *P < 0.05 and **P < 0.01 indicate the difference among the groups.