| Solution | Time (sec) | |
|---|---|---|
| 1 | 75% EtOH | 30 |
| 2 | RNase-free water | 30 |
| 3 | Hematoxylin | 40 |
| 4 | RNase-free water | 30 |
| 5 | 75% EtOH | 30 |
| 6 | 95% EtOH | 30 |
| 7 | 100% EtOH | 30 |
| 8 | Airdry | 120 |
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