Fig. 3.
Viewing of raw data and analysis of LDH kinetics results. (A) Microplate reader set up is illustrated with instructions for correct settings. (B) Students view the output from the microplate reader. (C) Initial rate velocities and enzyme function plots guided students through the analysis of results. Here, students were also given a chance to review concepts about enzyme structure and function. Graph-plotting software was used to calculate functional constants Vmax and Km. (D) Students are guided towards determining the type of enzyme inhibition.