Fig. S4.

C57BL Q97^CRE mice exhibit motoric and striatal neurodegenerative changes. The FVB-NJ genetic background was outbred for 10 successive matings using double-heterozygous BACHD⁺/CAG⁻CRE^ER+ offspring with WT C57BL mice. (A) At 9 mo of age, the motoric performance of tamoxifen-treated CTL, Q97, and Q97^CRE mice was examined with the balance beam test. Compared with controls, Q97^CRE and Q97 mice have significantly higher numbers of slips (mean ± SEM; mean comparisons were performed with the Kruskal–Wallis test, whereas post hoc analyses used Dunn’s multiple comparisons test). (B) At 12 mo of age, the striata of Q97 and Q97^CRE mice were examined with electron microscopy and exhibited enhanced neuronal degeneration (bars depict percentage ± 95% confidence interval; each comparison was made using the χ² test). *P < 0.05. (C and D) Representative normal and degenerating neurons in CTL and Q97^CRE specimens, respectively. (Scale bars: C and D represent 2 μm.)