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. 2016 May 3;113(20):5730–5735. doi: 10.1073/pnas.1520489113

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Fig. S3. — Immune cell analysis in VSV-infected mouse OB tissue by flow cytometry. (A) Gating strategy used for FACS and flow cytometry experiments: Cells were gated on live/dead, forward-scatter (FSC) singlets, side-scatter (SSC) singlets, TCRβ/CD19-negative, CD45/CD11b cells. Three cell populations distinguished based on CD45 and CD11b expression (P1, CD45^int/CD11b^hi; P2, CD45^hi/CD11b^hi; and P3, CD45^hi/CD11b^int) were each gated further on CD11c cells. (B) Three cell populations (P1, CD45^int/CD11b^hi; P2, CD45^hi/CD11b^hi; and P3, CD45^hi/CD11b^int) were analyzed by flow cytometry 24–96 hpi in control (PBS-treated) and VSV-infected mouse OB.