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Viral titer in the OB of VSV-infected mice. Total RNA was isolated from OB tissue at the indicated time points, and the VSV glycoprotein transcript was amplified using qPCR. The VSV titer (pfu) was calculated based on a logarithmic standard curve generated with known amounts of purified virus. Data are expressed as mean ± SEM and represent a composite of two independent experiments (total n = 4–5 per time point).
