Fig. 6.
LEC-derived IL-7 functions to maintain memory Th2 cells in the lung. (A) Effector Th2 cells from Ly5.1 OT-II Tg mice were transferred into Il-7fl/fl mice or Il-7fl/fl × Tie2-CreTg mice. Mice were challenged i.n. with OVA on days 1 and 3 and assessed on day 34. (B) Representative staining profiles of Ly5.1 and CD4 are shown (Left) with absolute cell numbers of memory Th2 cells (Ly5.1+) in the lungs (Right). The mean values from five mice per group are shown with SD. (C) Representative confocal micrographs of lung tissues stained with anti-B220 (green), anti-CD3ε (blue), and anti-Ly5.1 (red) are shown (Left). Quantification of lymphoid areas of the lungs were assessed by immunofluorescence microscopy (Right) (**P < 0.01; and *P < 0.05). (Scale bars, 40 μm.) Two independent experiments were performed with similar results (B and C).