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. 2016 May 2;113(20):E2842–E2851. doi: 10.1073/pnas.1512600113

Fig. 7.

Fig. 7.

Thy1+IL-7–producing LECs were increased in ectopic lymphoid tissue of nasal polyps from patients with ECRS. (A) Representative confocal micrograph of nasal polyp tissues from patients with ECRS and normal nasal mucosa stained with anti–HLA-DR (green), anti-CD3 (red), and TO-PRO3 (blue) (Left and Middle). Quantification analysis of aggregated lymphoid structures in nasal polyp tissues is shown (Right) (mean ± SD; n = 5 for normal mucosa, n = 9 for ECRS). (Scale bars, 100 μm.) (B) Representative confocal micrograph of nasal polyp tissues from patients with ECRS stained with anti-B220 (red), anti-CD4 (blue), and anti-CD11c (green) (Upper Left); anti-CD69 (red), anti-CD45RO (blue), and anti-CD4 (green) (Upper Right); anti-PECAM1 (green), anti-CD3 (red), and TO-PRO3 (blue) (Lower Left); or anti-HLA-DR (green), anti-CD4 (red), and anti-PECAM1 (blue) (Lower Right). Two independent experiments were performed with similar results. (Scale bars, 20 μm.) (C) Representative confocal micrographs of the nasal polyp tissue from patients with ECRS and normal nasal mucosa stained with anti-Podoplanin (green) and anti-CD3 (red) (Left and Middle), quantification analysis of Podoplanin+ lymphatics is shown (Right) (mean ± SD; n = 5 for normal mucosa, n = 10 for ECRS). (Scale bars, 100 μm.) (D) Representative staining profiles of CD45, PECAM1, and Thy1 on nasal polyp tissues from the patients with ECRS are shown. (E) Quantitative RT-PCR analysis of IL7 and IL33 expression in CD45−PECAM1+Thy1+ cells and CD45−PECAM1+Thy1− cells sorted from nasal polyp tissues from the patients with ECRS are shown. Three technical replicates were performed with quantitative RT-PCR. Two independent experiments were performed with similar results (D and E). (F) Representative confocal micrograph of the nasal polyp tissues from patients with ECRS stained with anti-Podoplanin (green), anti-CD3 (blue), and anti-Thy1 (red). (Scale bars, 20 μm.) Two independent experiments were performed with similar results (**P < 0.01; and *P < 0.05).