Table S1.
LN18 | U138MG | U251 | DBTRG | G5T | ||||||
Antibody | Parental | Sphere | Parental | Sphere | Parental | Sphere | Parental | Sphere | Parental | Sphere |
GM3 | ++ | + | ++ | + | − | − | − | − | − | − |
GM2 | +++ | +++ | ++++ | ++++ | ++++ | +++ | ++++ | ++ | ++ | ++ |
GM1 | +++ | +++ | ++++ | ++++ | ++++ | +++ | ++++ | ++ | ++ | ++ |
GD1a | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | +++ | + | +++ |
GD3 | + | ++ | − | + | − | ++ | − | ++ | − | +++ |
GD2 | + | +++ | − | + | − | ++ | − | ++++ | − | +++ |
GT1b | ++++ | ++++ | ++++ | ++++ | ++ | ++ | N.D. | N.D. | + | + |
A2B5 | ++ | ++ | ++ | ++ | + | + | + | + | + | + |
LeX | − | − | − | + | +++ | ++ | N.D. | +++ | +++ | +++ |
sLeX | − | − | − | − | ++ | − | N.D. | N.D. | − | − |
LeY | ++ | + | − | + | ++ | +++ | N.D. | N.D. | − | ++ |
TF | +++ | + | ++ | ++ | + | +++ | N.D. | N.D. | − | + |
Tn | +++ | ++ | + | ++ | ++ | + | N.D. | N.D. | + | + |
sTn | − | − | + | ++ | − | − | N.D. | N.D. | − | − |
SSEA3 | ++ | + | ++ | − | − | − | − | − | ++++ | +++ |
SSEA4 | ++++ | + | ++++ | + | +++ | − | ++++ | ++ | ++++ | ++++ |
Globo H | + | − | − | − | − | − | − | − | − | − |
CD44 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ |
CD24 | ++ | + | + | − | + | − | +++ | + | ++++ | + |
CD90 | ++++ | ++++ | ++++ | ++++ | ++++ | ++++ | ++ | ++ | ++++ | ++++ |
CD133 | − | − | − | − | − | − | − | + | − | − |
The intensity of glycan-related molecules was determined by flow cytometry as described in Materials and Methods. The symbols represent the percentage of positive cells in total cells: −, positive cells < 2.5%; +, 2.5% < positive cells < 25%; ++, 25% < positive cells < 50%; +++, 50% < positive cells < 75%; ++++, positive cells > 75%. N.D., not determined.