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. 2016 May 3;113(20):5592–5597. doi: 10.1073/pnas.1604721113

Table S1.

Expression profiles of glycan-related molecules in GBM parental cells and neurospheres

LN18 U138MG U251 DBTRG G5T
Antibody Parental Sphere Parental Sphere Parental Sphere Parental Sphere Parental Sphere
GM3 ++ + ++ +
GM2 +++ +++ ++++ ++++ ++++ +++ ++++ ++ ++ ++
GM1 +++ +++ ++++ ++++ ++++ +++ ++++ ++ ++ ++
GD1a ++++ ++++ ++++ ++++ ++++ ++++ ++++ +++ + +++
GD3 + ++ + ++ ++ +++
GD2 + +++ + ++ ++++ +++
GT1b ++++ ++++ ++++ ++++ ++ ++ N.D. N.D. + +
A2B5 ++ ++ ++ ++ + + + + + +
LeX + +++ ++ N.D. +++ +++ +++
sLeX ++ N.D. N.D.
LeY ++ + + ++ +++ N.D. N.D. ++
TF +++ + ++ ++ + +++ N.D. N.D. +
Tn +++ ++ + ++ ++ + N.D. N.D. + +
sTn + ++ N.D. N.D.
SSEA3 ++ + ++ ++++ +++
SSEA4 ++++ + ++++ + +++ ++++ ++ ++++ ++++
Globo H +
CD44 ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++ ++++
CD24 ++ + + + +++ + ++++ +
CD90 ++++ ++++ ++++ ++++ ++++ ++++ ++ ++ ++++ ++++
CD133 +

The intensity of glycan-related molecules was determined by flow cytometry as described in Materials and Methods. The symbols represent the percentage of positive cells in total cells: −, positive cells < 2.5%; +, 2.5% < positive cells < 25%; ++, 25% < positive cells < 50%; +++, 50% < positive cells < 75%; ++++, positive cells > 75%. N.D., not determined.