Fig. 2.

TPC2 expression level determines the melanin content in MNT-1 cells. (A) RNA was isolated from wild-type MNT-1 cells or two independent clones of CRISPR/Cas9 TPC2-KO homozygous cells and reverse-transcribed into cDNA. Relative amounts of the TPC2 mRNA were determined in triplicate by real-time PCR. (B) Melanin content was determined for the cells in A. (C) Melanin content was determined for mock-transfected wild-type MNT-1 cells, CRISPR/Cas9 TPC2-KO cells, and TPC2-GFP transfected TPC2-KO cells 5 d posttransfection (n = 2 for WT+Mock, n = 3 for TPC2-KO+Mock, and n = 9 for TPC2-KO+TPC2-GFP cells).