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. 2016 May 2;113(20):5622–5627. doi: 10.1073/pnas.1600108113

Fig. 4.

Fig. 4.

TPC2 regulates melanosome size. (A) Wild-type or CRISPR TPC2-KO MNT-1 cells expressing Cherry-Rab27a were analyzed by confocal fluorescence microscopy. Insets are magnifications of the boxed regions. (Scale bar, 5 µm.) (B) The average melanosome size was measured for wild-type and TPC2-KO cells (n = 37 and 41 cells, respectively). (C) Electron micrographs of wild-type (Left) or CRISPR TPC2-KO (Right) MNT-1 cells (18,500×). Roman numerals indicate melanosome maturation stage. (Scale bar, 500 nm.) (D) The average stage II–IV melanosome size was measured for wild-type and TPC2-KO cells (wild-type: seven cells, 464 melanosomes; TPC2-KO: nine cells, 567 melanosomes).