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Oligonucleotides used for sgRNA cloning into pX330 vector
| sgRNA name | Direction | Sequence 5′ to 3′ | Modification |
| sgRNA1 | F | caccgCGCGCACGGCACTGTGGCGC | TPC2-KO |
| R | aaacGCGCCACAGTGCCGTGCGCGc | ||
| sgRNA2 | F | caccgACCAGGTTCGAGTCCGAGCG | |
| R | aaacCGCTCGGACTCGAACCTGGTc | ||
| sgRNA3 | F | caccgGGCGCGCCCGGCAGAAAGCG | |
| R | aaacCGCTTTCTGCCGGGCGCGCCc | ||
| sgRNA4 | F | caccgTAGTGGCTACATCCATCCTG | TPC2-EmGFP |
| R | aaacCAGGATGGATGTAGCCACTAc | ||
| sgRNA5 | F | caccgCCAGCTTAGTGGTCCGTCAG | |
| R | aaacCTGACGGACCACTAAGCTGGc |
