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. 2016 Apr 28;13(6):5045–5052. doi: 10.3892/mmr.2016.5204

Figure 1.

Figure 1

TSA has a synergistic inhibitory effect on genistein-induced HEp-2 cell growth impairment. An MTT assay was used to detect the effects of genistein and TSA on HEp-2 cell viability (A) individually and (B–F) in combination, to determine their synergistic effects at indicated measuring time points. The HEp-2 cells treated with genistein and TSA alone and in combination were stained with EdU. The nuclei were also visualized using Hoechst 33342 staining. The percentage of cell proliferation is expressed as the ratio of EdU-positive cells to total Hoechst 33342-positive cells. The number of untreated cells was set as 100%. Magnification, ×200. (G) Representative images showing the effects of TSA, genistein and TSA+genistein on HEp-2 single cell colony formation, with (H) quantification presented. All data were obtained from at least three independent experiments and are presented as the mean ± standard error of the mean. *P<0.05, **P<0.01 and ***P<0.001, vs. Mock. TSA, trichostatin A.