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. 2016 Apr 28;13(6):5045–5052. doi: 10.3892/mmr.2016.5204

Figure 3.

Figure 3

Effects of combined TSA and genistein treatment on HEp-2 cells apoptosis. Following treatment for 48 h with TSA, genistein or TSA+genistein, the HEp-2 cells exhibited a (A) pro-apoptotic phenotype (magnification, ×200) with (B) PPARγ activation, detected using western blotting. Fluorescence-activated cell sorting analysis with (C and D) PI and Annexin VI staining and a (E and F) TUNEL assay (magnification, ×200) were utilized to examine the combined effects of TSA+genistein on HEp-2 apoptosis. The data were obtained from three independent experiments and expressed as the mean ± standard error of the mean. *P<0.05, **P<0.01 and ***P<0.001, vs. Mock. TSA, trichostatin A; PPARγ, peroxisome proliferator-activated receptor γ; PI, propidium iodide; FITC, fluorescein isothiocyanate.