Figure 4.

Effects of combined treatment of TSA and genistein on Akt signaling. The HEp-2 cells were incubated with TSA, genistein or TSA+genistein for 48 h, and western blotting was used to examine the (A) activity of the Akt pathway by the levels of phosphorylated (p) Akt, S6 and 4eBP1. (B) Apoptotic-associated protein expression of Bcl-2 and Bax. Quantitative measurements were obtained from 3 independent experiments and are presented as the mean ± the standard error of the mean. ^*P<0.05, ^**P<0.01 and ^***P<0.001 vs. Mock. TSA, TSA, trichostatin A; Bcl-2, B cell lymphoma-2; Bax, Bcl-2-associated X protein.