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. 2016 Apr 13;13(6):4585–4592. doi: 10.3892/mmr.2016.5123

Figure 2.

Figure 2

Expression of ASK1-associated downstream targets in the brain of the rat HI model. (A and B) Western blotting detection of p-JNK and JNK expression in the sham and HI model at 6, 12, 24 and 48 h after brain insult. GAPDH was used as a loading control (n=4; **P<0.01). (C) Immunofluorescence detection of p-JNK in the brain of the sham and HI model at 6, 12, 24 and 48 h after brain insult. DAPI was used to indicate the cell nucleus (magnification, ×200). (D and E) Western blotting detection of p-c-Jun, c-Jun, p53 and Caspase 3 in the sham and HI model at 6, 12, 24 and 48 h after brain insult. GAPDH was used as a loading control (n=4; **P<0.01). ASK, apoptosis signal-regulating kinase 1; HI, hypoxia-ischemia; DAPI, 4′,6-diamidino-2-phenylindole; GFAP, glial fibrillary acidic protein.