Table I.

Primer design of semiquantitative RT-PCR validation.

Gene symbol	Ref Seq ID	Forward primer	Reverse primer
GPNMB	NM_002510	5′-CCTCGTGGGCTCAAATATAACAT-3′	5′-ACTGTCCTCTGACCATGCTGT-3′
CTSK	NM_000396	5′-ACTCAAAGTACCCCTGTCTCAT-3′	5′-CCACAGAGCTAAAAGCCCAAC-3′
IGFBP6	NM_002178	5′-GAGGGGCTCAAACACTCTACG-3′	5′-CCATCCGATCCACACACCA-3′
NT5E	NM_002526	5′-CCAGTACCAGGGCACTATCTG-3′	5′-TGGCTCGATCAGTCCTTCCA-3′
MARC1	NM_022746	5′-CCTCGCCTGGTCCTGATTTC-3′	5′-GGCAGTAGTAGGTCCTTTGTGT-3′
LGALS12	AF222694	5′-AACCCTCGCTTCCATACCAC-3′	5′-TCCTCATTCCCGAAGAGAAAGAG-3′
Spot14	AW272342	5′-CAGGTGCTAACCAAGCGTTAC-3′	5′-CAGAAGGCTGGGGATCATCA-3′

Primer design of the genes identified on microarray that were randomly selected for RT-PCR validation. RT-PCR, reverse transcription-polymerase chain reaction; GPNMB, glycoprotein nmb; CTSK, cathepsin K; IGFBP6, insulin like growth factor binding protein 6; NT5E, 5′-nucleotidase ecto; MARC1, mitochondrial amidoxime reducing component 1; LGALS12, lectin, galactoside-binding, soluble, 12; Spot14 (or THRSP), thyroid hormone responsive.