Figure 6.

Proliferation of ASMCs induced by CSE (5%). (A) DNA synthesis in ASMCs stimulated with CSE (5%) for 24 h was assessed by methyl-[³H]-thymidine incorporation. (B) Cell number of ASMCs stimulated with CSE (5%) for 24 h was detected by hemocytometer. Data are presented as the mean ± standard error of the mean of eight independent experiments. ^*P<0.05 vs. ASMCs without lentivirus vector treatment. CSE, cigarette smoke extract; TRPM7, transient receptor potential cation channel subfamily M member 7; shRNA, short hairpin RNA; ASMCs, airway smooth muscle cells.